Western blots of rhPln.D1 glycosidase digests. Pooled anionic exchange fractions (34-52 ml) from HEK 293 culture (Pln.198) or HUVECs (Pln.247) were subjected to digestion with either buffer only, heparinase I (Hep I), heparinase II (Hep II), heparinase III (Hep III), chondroitinase ABC (Chond. abc), all three heparinases plus chondroitinase ABC, or heparanase. Treated samples were analyzed by western blot using mAb CSI 001-71. Similar data were generated using plasmid expression of pln.247 (not shown).