Figure 6
From: Sequencing bias: comparison of different protocols of MicroRNA library construction
Fold difference of miR-1 and miR-206 between Hu sheep and Dorper sheep. Fold differences of muscle-specific miRNAs, miR-1 and miR-206, between Hu sheep and Dorper sheep were assessed amongst cloning and SBS sequencing data and qRT-PCR. Fold differences for qRT-PCR data were calculated by ΔΔCT between Hu sheep and Dorper sheep, where ΔΔCT = ΔCTmiRNA-ΔCTmiR-16. Fold differences for sequencing data were calculated by the equation: log2 (ratio of reads count of Hu sheep/ratio of reads count of Dorper sheep).