Figure 5
From: Sequencing bias: comparison of different protocols of MicroRNA library construction
Fold difference of qRT-PCR and Illumina GA data between Hu sheep and Dorper sheep. Fold difference between Hu sheep and Dorper sheep of qRT-PCR (grey) and Illumina GA data (black) for 9 miRNAs are shown. Fold differences for qRT-PCR data were calculated by ΔΔCT between Hu sheep and Dorper sheep, where ΔΔCT = ΔCTmiRNA-ΔCTmiR-16. Fold differences for Illumina GA data were calculated by the equation: log2 (ratio of read count of Hu sheep/ratio of read count of Dorper sheep).