Figure 4
From: Sequencing bias: comparison of different protocols of MicroRNA library construction
qRT-PCR and SOLiD cloning data for Hu sheep and Dorper sheep. The relative abundance of 9 miRNAs calculated by qRT-PCR (red) and SOLiD cloning sequencing (green) for Hu sheep (A) and Dorper sheep (B) are shown. Relative abundance of the miRNAs was calculated by the 2-ΔΔCT method using miR-16 as reference to normalize the results. Relative abundance of the miRNAs for the SOLiD cloning data was calculated by the frequency of reads count of each miRNA.