TY - JOUR AU - Tian, Geng AU - Yin, XuYang AU - Luo, Hong AU - Xu, XiaoHong AU - Bolund, Lars AU - Zhang, XiuQing PY - 2010 DA - 2010/09/06 TI - Sequencing bias: comparison of different protocols of MicroRNA library construction JO - BMC Biotechnology SP - 64 VL - 10 IS - 1 AB - MicroRNAs(miRNAs) are 18-25 nt small RNAs playing critical roles in many biological processes. The majority of known miRNAs were discovered by conventional cloning and a Sanger sequencing approach. The next-generation sequencing (NGS) technologies enable in-depth characterization of the global repertoire of miRNAs, and different protocols for miRNA library construction have been developed. However, the possible bias between the relative expression levels and sequences introduced by different protocols of library preparation have rarely been explored. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-10-64 DO - 10.1186/1472-6750-10-64 ID - Tian2010 ER -