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Table 2 LC-MS/MS analysis of in-gel-chymotrypsin-digested LTBMUC1 produced in N. benthamiana L. plants co-expressing human GalNAc-T2, Y. enterocolitica UDP-GlcNAc-4 epimerase and C. elegans UDP-GlcNAc/UDP-GalNAc transporter.

From: Engineering of N. benthamianaL. plants for production of N-acetylgalactosamine-glycosylated proteins - towards development of a plant-based platform for production of protein therapeutics with mucin type O-glycosylation

Peptide sequence

MH+

Xcorr

dCn

(Y)LTETKIDKL(C)

1060.80

2.69

0.61

(W)NNKTPNSIAAI(S)

1142.84

2.61

0.84

(W)NNKTPNSIAAISM(S)

1361.75

2.37

0.64

(Y)TINDKILSY(T)

1066.36

1.99

0.73

  1. The amino acids in parenthesis at the beginning and end of each peptide show the flanking residue in the entire protein sequence.