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Table 1 LC-MS/MS analysis of in-gel chymotrypsin-digested N. benthamiana L. 33 kDa endogenous polypeptide band identified as endochitinase (EC 3.2.1.14, GenBank Accession No P24091).

From: Engineering of N. benthamianaL. plants for production of N-acetylgalactosamine-glycosylated proteins - towards development of a plant-based platform for production of protein therapeutics with mucin type O-glycosylation

Peptide sequence

MH+

Xcorr

dCn

(Y)C*SILGVSPGDNLDC*GNQRSF(G)

2197.29

4.24

0.12

(F)FAQTSHETTGGWATAPDGPY(A)

2095.17

3.98

0.77

(L)NNPDLVATDPVISF(K)

1502.65

3.68

0.79

(F)AQTSHETTGGWATAPDGPY(A)

1948.00

3.56

0.85

(L)GVSPGDNLDC*GNQRSFGN(G)

1894.91

3.54

0.44

(F)FAQTSHETTGGW(A)

1322.37

3.48

0.85

(L)GVSPGDNLDC*GNQRSF(G)

1723.76

3.30

0.31

(Y)NYGPC*GRAIGVDLL(N)

1505.67

3.19

0.60

(Y)NAFINAARSFPGFGTSGD(T)

2032.95

2.94

0.73

(F)INAARSFPGF(G)

1080.22

2.80

0.60

(N)RLPGFGVITN(I)

1074.26

2.69

0.85

(F)GRGPIQISHNY(N)

1242.37

2.67

0.79

(L)LNNPDLVATDPVISF(K)

1615.81

2.63

0.69

(Y)FGRGPIQISHNY(N)

1389.54

2.57

0.85

(V)ITNIINGGLEC*GRG(T)

1474.62

2.51

0.78

(A)FINAARSFPGFGTSGD(T)

1847.77

2.37

0.65

(F)INAARSFPGFGTSGD(T)

1700.59

2.13

0.84

  1. The threonine residue (Thr-119) modified by 203 amu is in boldface type, carboxymethylated cysteine residues are denoted by asterisks. The amino acids in parenthesis at the beginning and end of each peptide show the flanking residue in the entire protein sequence.