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Figure 5 | BMC Biotechnology

Figure 5

From: Engineering of N. benthamianaL. plants for production of N-acetylgalactosamine-glycosylated proteins - towards development of a plant-based platform for production of protein therapeutics with mucin type O-glycosylation

Figure 5

Glycosylation of endogenous N. benthamiana L. proteins upon ectopic expression of human GalNAc-T2. A - VVA-lectin blot of the soluble proteins bound to VVA-agarose from control (C) and transgenic (GalNAc-T2) plants, and the corresponding silver staining for the GalNAc-T2 plant sample; B - sequence of tobacco endochitinase (GenBank Accession No P24091) identified with the 33 kDa band denoted in A by an asterisk: the signal peptide is underlined, the propeptide is double underlined, the covered regions after LC-MS/MS analysis of the chymotryptic fragments are gray-highlighted, the modified Thr-119 by 203 amu is red-highlighted; C - MS/MS analysis confirming the attachment of GalNAc (203 amu) to Thr-119; D - homology model of tobacco endochitinase built based on the 3 D structure of O. sativa L. japonica class I chitinase (pdb ID 2DKV); the position of the glycosylated Thr-119 is denoted by halos.

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