Figure 6

Binding of TCR-Ig to specific pMHC expressing cells. A20 cells (top panel), A20 cells loaded with synthetic peptide (A20 pept, second panel), F9 cells (third panel), and F9 cells loaded with synthetic peptide (F9 pept, forth panel) were stained with the 4B2A1-based purified cTCR-cIg protein (4mutSS, 100 μg/ml, all left panels) and the 7A10B2-based cTCR-cIg protein (7wtSS, 17 μg/ml, all right panels). Thin line, PBS control; dotted line, NIP-specific Ab (isotype matched); thick line: cTRC-cIg fusion protein; broken line, I-E-specific Ab (isotype-matched). In each case, 10⁶ cells were stained after pulsing twice with 50 μM peptide, both 18 and 2 hours before the experiment was carried out. One representative experiment out of three is shown.