Figure 4
From: Stabilizing mutations increase secretion of functional soluble TCR-Ig fusion proteins
Effect of V and C region mutations on secretion and folding of cTCR-cIg molecules produced in HEK 293E cells. Secretion of molecules with or without V region mutations (4 mut and 7 mut). One representative experiment out of three is shown (A). Secretion of molecules with or without V region mutations and C regions with an introduced disulfide bridge (denoted SS and mutSS). One representative experiment out of three is shown (B). Recognition by anti-TCR Abs. C. The 4B2A1 derived fusions were tested for binding to 4B2A1 specific mAbs. One representative experiment out of two is shown (C and D). The 7A10B2 derived fusions were tested for binding to the 7A10B2 specific mAbs. H57 is Cβ region specific. One representative experiment out of two is shown (D). Cell supernatant was tested in triplicates. Error bars: SD