Table 2 Summary of the absorption and fluorescence emission data relevant to the evaluation of the extinction coefficient εmax using the Strickler-Berg equation.
From: A new approach to dual-color two-photon microscopy with fluorescent proteins
Protein | λMax emission (nm) | τ (ns) | Quantum Yield (ϕ) | τR | λMax absorption (nm) | εMAX 103M-1cm-1 |
|---|---|---|---|---|---|---|
mTFP1.0 | 494 | 3.2 | 0.87 ± 0.09 | 3.7 | 463 | 49.6 |
mTFP1.0b | 492 | 3.14 | 0.88 ± 0.09 | 3.5 | 462 | 48.1 |
mTFP0.8 | 494 | 3.63 | 0.91 ± 0.09 | 3.8 | 471 | 46.5 |
mTFP0.7 | 489 | 1.82 | 0.48 ± 0.05 | 3.8 | 453 | 43.4 |
G1 | 504 | 3.29 | 0.83 ± 0.12 | 4.0 | 490 | 71.2 |
G3 | 512 | 2.99 | 0.73 ± 0.07 | 4.1 | 498 | 68.0 |
mWasabi | 508 | 3.4 | 0.81 ± 0.08 | 4.2 | 498 | 73.2 |
mKalama1 | 455 | 1.84 | 0.35 ± 0.07 | 4.7 | 392 | 25.7 |
mAmetrine | 525 | 3.93 | 0.57 ± 0.13 | 7.5 | 407 | 28.7 |
EBFP2 | 446 | 3.65 | 0.64 ± 0.13 | 5.3 | 390 | 24.0 |
EBFP1.5 | 448 | 2.4 | 0.43 ± 0.09 | 6.2 | 389 | 23.5 |
EBFP1.2 | 443 | 2.16 | 0.36 ± 0.07 | 5.7 | 386 | 21.8 |
mCerulean | 477 | 3.42 | 0.55 ± 0.05 | 6.2 | 431 | 25.1 |
ECFP(N164H) | 476 | 3.36 | 0.49 ± 0.05 | 6.9 | 433 | 22.7 |