Table 1 Summary of the optimum two-photon excitation wavelengths and corresponding peak cross section and peak brightness values (per chromophore) of the FPs studied.
From: A new approach to dual-color two-photon microscopy with fluorescent proteins
Protein | λopt (nm) | σ2 (λopt )GM | σ2ϕ (λopt )GM |
|---|---|---|---|
mTFP1.0 | 867 | 75 | 65 |
mTFP1.0b | 871 | 75 | 66 |
mTFP0.8 | 885 | 65 | 59 |
mTFP0.7 | 865 | 84 | 40 |
G1 | 918 | 63 | 52 |
G3 | 937 | 40 | 29 |
mWasabi | 927 | 43 | 35 |
mKalama1 | 770 | 30 | 11 |
mAmetrine | 809 | 45 | 26 |
EBFP2 | 750 | 12 | 7.7 |
EBFP1.5 | 750 | 10 | 4.3 |
EBFP1.2 | 750 | 11 | 4.0 |
ECFP(N164H) | 857 | 23 | 11 |
mCerulean | 858 | 23 | 13 |