Figure 1
From: A new approach to dual-color two-photon microscopy with fluorescent proteins
Two-photon cross section and brightness of blue/teal/green FP series. 2PA spectra (red circles), one-photon fluorescence excitation spectra (blue line), and fluorescence emission spectra (black line) of the FPs studied. The left-ordinate axis represents the two-photon cross section values (GM), and the right-ordinate axis represents the two-photon brightness, σ2φ, (GM). The bottom x-axis represents the laser wavelength used for excitation and the top x-axis represents the transition wavelength. The excitation and fluorescence emission intensities are shown in arbitrary units.