Skip to main content

Table 1 Cell viability tests on Tera-1 and HEK-293 cells.

From: Novel strong tissue specific promoter for gene expression in human germ cells

Experimenta

Tera-1

HEK-293

 

PI assayb

Annexin Vc

PI assayb

Annexin Vc

-5FC; -Transf.

9.2 ± 2.3

ND

7.7 ± 1.5

ND

-5FC; no-RCD

9.0 ± 1.7

ND

7.4 ± 1.7

ND

-5FC; mNUS-RCD

8.8 ± 2.9

ND

7.8 ± 2.1

ND

-5FC; CMV-RCD

9.9 ± 2.5

ND

8.2 ± 2.2

ND

+5FC; -Transf.

9.4 ± 2.1

10.9 ± 3.3

7.4 ± 1.8

8.7 ± 2.3

+5FC; no-RCD

9.1 ± 2.5

10.7 ± 2.6

7.7 ± 1.6

8.6 ± 2.9

+5FC; mNUS-RCD

59.5 ± 6.4

59.1 ± 3.9

7.8 ± 1.7

8.4 ± 2.8

+5FC; CMV-RCD

67.3 ± 7.5

64.9 ± 5.6

73.8 ± 11.3

75.5 ± 8.6

  1. aCell viability tests shown on this table were done 24 hours after application of 5FC. "-5FC" means "no 5FC was added", "+FC" marks experiments with the addition of 5FC to the medium. "-Transf.", "no-RCD", "mNUS-RCD" and "CMV-RCD" mean "no transfection", "transfection with no-RCD construct", "transfection with mNUS-RCD" and "transfection with CMV-RCD", accordingly.
  2. bPropidium iodide tests were done at least in quadruplicates. The data represent integral proportion of necrotic and apoptotic cells.
  3. cAnnexin V assay was performed for all the experiments with the addition of 5FC to the medium. Tests were done in triplicates. The data represent integral proportion of necrotic and apoptotic cells.