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Table 1 Cell viability tests on Tera-1 and HEK-293 cells.

From: Novel strong tissue specific promoter for gene expression in human germ cells

Experimenta Tera-1 HEK-293
  PI assayb Annexin Vc PI assayb Annexin Vc
-5FC; -Transf. 9.2 ± 2.3 ND 7.7 ± 1.5 ND
-5FC; no-RCD 9.0 ± 1.7 ND 7.4 ± 1.7 ND
-5FC; mNUS-RCD 8.8 ± 2.9 ND 7.8 ± 2.1 ND
-5FC; CMV-RCD 9.9 ± 2.5 ND 8.2 ± 2.2 ND
+5FC; -Transf. 9.4 ± 2.1 10.9 ± 3.3 7.4 ± 1.8 8.7 ± 2.3
+5FC; no-RCD 9.1 ± 2.5 10.7 ± 2.6 7.7 ± 1.6 8.6 ± 2.9
+5FC; mNUS-RCD 59.5 ± 6.4 59.1 ± 3.9 7.8 ± 1.7 8.4 ± 2.8
+5FC; CMV-RCD 67.3 ± 7.5 64.9 ± 5.6 73.8 ± 11.3 75.5 ± 8.6
  1. aCell viability tests shown on this table were done 24 hours after application of 5FC. "-5FC" means "no 5FC was added", "+FC" marks experiments with the addition of 5FC to the medium. "-Transf.", "no-RCD", "mNUS-RCD" and "CMV-RCD" mean "no transfection", "transfection with no-RCD construct", "transfection with mNUS-RCD" and "transfection with CMV-RCD", accordingly.
  2. bPropidium iodide tests were done at least in quadruplicates. The data represent integral proportion of necrotic and apoptotic cells.
  3. cAnnexin V assay was performed for all the experiments with the addition of 5FC to the medium. Tests were done in triplicates. The data represent integral proportion of necrotic and apoptotic cells.