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Figure 6 | BMC Biotechnology

Figure 6

From: Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection

Figure 6

Small scale purification of expression products. The cells of 4 ml culture were pelleted and disrupted by sonification. After centrifugation the target proteins in the supernatant were purified by automated batch IMAC and run on 12% SDS-PAGE. In each panel, total protein T, protein in the supernatant S and purified protein P are given. Typical examples of purifications are shown.

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BMC Biotechnology

ISSN: 1472-6750

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