Figure 4From: Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selectionHigh expression levels are reached with most of the vectors. The cell pellets from expression cultures were extracted with SDS-gel sample buffer and run on 12% SDS_PAGE. From left to right: Mr markers; 1, PSTCm1-EB1; 2, PSPCm2-VP3; 3, PSTCm8-CapGly; 4, PSTCm8-TTL; 5, PSTCm11-CapGly; 6, PSKn1-Clip170; 7, NSkn1-EB1. The desired expression product is represented by the strongest band in the corresponding lane with the total cell extract. Only the vector PSAp1 gave less vigorous expression (not shown).Back to article page