Table 1 DNA measurements and GMO values (% RRS) for all DNA extraction method/matrix combinations.
Extraction method | Matrix | 260:280 nm ratio | 260:230 nm ratio | Spectrophotometric DNA yield (μg g-1) | Inhibition run | % RRS | Fluorescence DNA yield (μg g-1) |
|---|---|---|---|---|---|---|---|
CTAB | Feed | 1.89 | 2.42 | 114.0 | Ok | 0.90 | 84.5 |
Feed | 1.87 | 2.39 | 133.5 | Ok | 0.88 | 101.0 | |
Feed | 1.88 | 2.34 | 127.0 | Ok | 0.78 | 99.0 | |
Feed | 1.86 | 2.42 | 121.0 | Ok | 0.63 | 94.0 | |
Feed | 1.87 | 2.35 | 144.0 | Out of AC* | 0.82 | 114.0 | |
Feed | 1.89 | 2.42 | 103.5 | Ok | 1.11 | 80.0 | |
Biscuit | 1.6 | 1.64 | 28.5 | Ok | 1.74 | 7.5 | |
Biscuit | 1.38 | 1.32 | 12.5 | Ok | 1.55 | 3.0 | |
Biscuit | 1.41 | 1.17 | 10.0 | Out of AC | 2.04 | 3.0 | |
Biscuit | 1.63 | 1.63 | 25.5 | Ok | 1.19 | 6.5 | |
Biscuit | 1.58 | 1.71 | 24.5 | Ok | 1.40 | 7.5 | |
Biscuit | 1.3 | 1.10 | 10.0 | Ok | 1.32 | 2.5 | |
CRM 1% | 1.67 | 1.87 | 69.5 | Ok | 1.31 | 56.0 | |
CRM 1% | 1.70 | 1.90 | 45.5 | Ok | 1.34 | 38.5 | |
CRM 1% | 1.73 | 2.15 | 65.0 | Ok | 1.01 | 59.0 | |
CRM 1% | 1.69 | 1.92 | 43.0 | Ok | 1.14 | 36.0 | |
CRM 1% | 1.69 | 1.95 | 41.0 | Ok | 1.45 | 47.5 | |
CRM 1% | 1.73 | 2.05 | 67.0 | Ok | 1.09 | 81.0 | |
Wizard | Feed | 1.70 | 0.87 | 83.0 | Out of AC | 1.31 | 32.5 |
Feed | 1.77 | 0.67 | 105.5 | Out of AC | 0.96 | 38.0 | |
Feed | 1.66 | 0.92 | 118.0 | Out of AC | 1.50 | 33.5 | |
Feed | 1.86 | 0.27 | 70.0 | Ok | 1.07 | 37.5 | |
Feed | 1.79 | 1.05 | 49.0 | Out of AC | 1.35 | 24.8 | |
Feed | 1.72 | 1.10 | 68.0 | Out of AC | 1.35 | 28.3 | |
Biscuit | 1.77 | 0.39 | 92.0 | Ok | 3.29 | 4.1 | |
Biscuit | 1.84 | 0.13 | 11.5 | Ok | 3.06 | 1.7 | |
Biscuit | 1.86 | 0.18 | 30.5 | Ok | 4.90 | 2.8 | |
Biscuit | 1.83 | 0.34 | 64.0 | Out of AC | 3.15 | 2.6 | |
Biscuit | 1.8 | 0.31 | 84.0 | Ok | 2.03 | 3.2 | |
Biscuit | 1.86 | 0.17 | 62.5 | Ok | 2.11 | 3.4 | |
CRM 1% | 1.59 | 0.23 | 62.0 | Out of AC | 1.04 | 3.7 | |
CRM 1% | 2.02 | 0.21 | 46.5 | Ok | 1.45 | 10.9 | |
CRM 1% | 1.98 | 0.17 | 56.5 | Ok | 0.58 | 4.1 | |
CRM 1% | 2.09 | 0.04 | 14.5 | Ok | 1.08 | 5.4 | |
CRM 1% | 1.87 | 0.27 | 86.5 | Out of AC | 1.34 | 9.6 | |
CRM 1% | 1.91 | 0.08 | 29.0 | Ok | 1.31 | 12.2 | |
Dellaporta | Feed | 2.19 | 0.18 | 72.0 | Out of AC | 0.99 | 10.3 |
Feed | 2.05 | 0.27 | 111.0 | Ok | 0.90 | 24.8 | |
Feed | 2.07 | 0.27 | 107.5 | Ok | 0.94 | 22.8 | |
Feed | 1.94 | 0.61 | 294.0 | Out of AC | 0.80 | 61.0 | |
Feed | 2.17 | 0.2 | 78.5 | Ok | 0.86 | 14.8 | |
Feed | 2.75 | 0.09 | 34.5 | Ok | 0.71 | 7.8 | |
Biscuit | 2.27 | 0.11 | 62.5 | Ok | 1.15 | 4.5 | |
Biscuit | 2.87 | 0.05 | 29.0 | Ok | 1.13 | 4.2 | |
Biscuit | 2.39 | 0.08 | 43.5 | Ok | 1.26 | 3.0 | |
Biscuit | 2.83 | 0.05 | 29.5 | Ok | 1.40 | 4.0 | |
Biscuit | 2.58 | 0.06 | 32.0 | Ok | 0.96 | 3.0 | |
Biscuit | 2.58 | 0.06 | 34.5 | Ok | 1.01 | 4.0 | |
CRM 1% | 1.90 | 2.11 | 74.0 | Ok | 1.03 | 42.5 | |
CRM 1% | 1.91 | 2.25 | 78.5 | Ok | 1.14 | 33.0 | |
CRM 1% | 1.71 | 1.89 | 21.5 | Out of AC | 0.93 | 15.0 | |
CRM 1% | 1.58 | 0.73 | 40.5 | Out of AC | 1.06 | 17.7 | |
CRM 1% | 1.78 | 4.25 | 45.0 | Ok | 1.25 | 22.2 | |
CRM 1% | 1.84 | 1.94 | 20.0 | Out of AC | 1.25 | 2.8 |