Skip to main content

Advertisement

Table 1 DNA measurements and GMO values (% RRS) for all DNA extraction method/matrix combinations.

From: Testing the interaction between analytical modules: an example with Roundup Ready®soybean line GTS 40-3-2

Extraction method Matrix 260:280 nm ratio 260:230 nm ratio Spectrophotometric DNA yield (μg g-1) Inhibition run % RRS Fluorescence DNA yield (μg g-1)
CTAB Feed 1.89 2.42 114.0 Ok 0.90 84.5
  Feed 1.87 2.39 133.5 Ok 0.88 101.0
  Feed 1.88 2.34 127.0 Ok 0.78 99.0
  Feed 1.86 2.42 121.0 Ok 0.63 94.0
  Feed 1.87 2.35 144.0 Out of AC* 0.82 114.0
  Feed 1.89 2.42 103.5 Ok 1.11 80.0
  Biscuit 1.6 1.64 28.5 Ok 1.74 7.5
  Biscuit 1.38 1.32 12.5 Ok 1.55 3.0
  Biscuit 1.41 1.17 10.0 Out of AC 2.04 3.0
  Biscuit 1.63 1.63 25.5 Ok 1.19 6.5
  Biscuit 1.58 1.71 24.5 Ok 1.40 7.5
  Biscuit 1.3 1.10 10.0 Ok 1.32 2.5
  CRM 1% 1.67 1.87 69.5 Ok 1.31 56.0
  CRM 1% 1.70 1.90 45.5 Ok 1.34 38.5
  CRM 1% 1.73 2.15 65.0 Ok 1.01 59.0
  CRM 1% 1.69 1.92 43.0 Ok 1.14 36.0
  CRM 1% 1.69 1.95 41.0 Ok 1.45 47.5
  CRM 1% 1.73 2.05 67.0 Ok 1.09 81.0
Wizard Feed 1.70 0.87 83.0 Out of AC 1.31 32.5
  Feed 1.77 0.67 105.5 Out of AC 0.96 38.0
  Feed 1.66 0.92 118.0 Out of AC 1.50 33.5
  Feed 1.86 0.27 70.0 Ok 1.07 37.5
  Feed 1.79 1.05 49.0 Out of AC 1.35 24.8
  Feed 1.72 1.10 68.0 Out of AC 1.35 28.3
  Biscuit 1.77 0.39 92.0 Ok 3.29 4.1
  Biscuit 1.84 0.13 11.5 Ok 3.06 1.7
  Biscuit 1.86 0.18 30.5 Ok 4.90 2.8
  Biscuit 1.83 0.34 64.0 Out of AC 3.15 2.6
  Biscuit 1.8 0.31 84.0 Ok 2.03 3.2
  Biscuit 1.86 0.17 62.5 Ok 2.11 3.4
  CRM 1% 1.59 0.23 62.0 Out of AC 1.04 3.7
  CRM 1% 2.02 0.21 46.5 Ok 1.45 10.9
  CRM 1% 1.98 0.17 56.5 Ok 0.58 4.1
  CRM 1% 2.09 0.04 14.5 Ok 1.08 5.4
  CRM 1% 1.87 0.27 86.5 Out of AC 1.34 9.6
  CRM 1% 1.91 0.08 29.0 Ok 1.31 12.2
Dellaporta Feed 2.19 0.18 72.0 Out of AC 0.99 10.3
  Feed 2.05 0.27 111.0 Ok 0.90 24.8
  Feed 2.07 0.27 107.5 Ok 0.94 22.8
  Feed 1.94 0.61 294.0 Out of AC 0.80 61.0
  Feed 2.17 0.2 78.5 Ok 0.86 14.8
  Feed 2.75 0.09 34.5 Ok 0.71 7.8
  Biscuit 2.27 0.11 62.5 Ok 1.15 4.5
  Biscuit 2.87 0.05 29.0 Ok 1.13 4.2
  Biscuit 2.39 0.08 43.5 Ok 1.26 3.0
  Biscuit 2.83 0.05 29.5 Ok 1.40 4.0
  Biscuit 2.58 0.06 32.0 Ok 0.96 3.0
  Biscuit 2.58 0.06 34.5 Ok 1.01 4.0
  CRM 1% 1.90 2.11 74.0 Ok 1.03 42.5
  CRM 1% 1.91 2.25 78.5 Ok 1.14 33.0
  CRM 1% 1.71 1.89 21.5 Out of AC 0.93 15.0
  CRM 1% 1.58 0.73 40.5 Out of AC 1.06 17.7
  CRM 1% 1.78 4.25 45.0 Ok 1.25 22.2
  CRM 1% 1.84 1.94 20.0 Out of AC 1.25 2.8
  1. * 'Out of AC' stands for data outside the acceptance criteria for DNA quality from inhibition runs