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Figure 5 | BMC Biotechnology

Figure 5

From: SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradation

Figure 5

Differential breakdown of GvpC and SIVsm components demonstrated by western blot. Monolayer samples were harvested at different times post application of gas vesicles, then mixed with 5× SDS-PAGE sample buffer and subsequently assessed through western blot detection of GvpC or SIVsm material as described in the methods. These representative western blots demonstrate that at both the 96 and 120 h times examined, strong bands detecting GvpC are evident. For the much smaller SIV component of the same gas vesicles, numerous weak bands were evident at the 96 h time but by 120 h, the bands detected were very light and evident only at molecular weights ≤ 14 kDa.

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