Intracellular degradation of GvpC specific protein of gas vesicles. Companion J774A.1 monolayers were prepared under conditions identical to those used for samples in figure 3 and similarly examined microscopically. This panel, immunostained with rabbit anti-GvpC antibody, demonstrates this gas vesicle specific protein also disappears over time. The quantities of GvpC protein remaining at the 120 h time appear greater than that observed for the SIVsm inserts. This would be consistent with the larger size of this protein and likely would support continued perturbation of cells following particle phagocytosis. Original magnification 1000 ×.