Figure 1From: SIVsm Tat, Rev, and Nef1: functional characteristics of r-GV internalization on isotypes, cytokines, and intracellular degradationPredominant Isotype Identification. Isotypes of sera collected at different post booster or re-immunization times were determined using sera pre-adsorbed with wt-gas vesicles and assessed using isolated recombinant GV as the SIVsm antigen source. As detailed in the methods, sera diluted to the pre-established optimum were incubated in the r-GV pre-coated wells. Following rinses and incubation with pre-conjugated isotype specific antibody, isotype specific binding was detected by addition of pNPP substrate and absorbances quantified at 405 nm.Back to article page