Dissociation and Quantification of the chimerical tobacco line T17. A) Dissociation of the chimerical tobacco line T17 on rooting medium. Explants were transformed with the binary vector pBin19-35SGusintron carrying the nptII transgene for kanamycin selection and screened phenotypically. Shoots (a) in the left-hand jar were chosen for their poor root system, light green leaves and bleaching symptoms, whereas shoots (b) in the right-hand jar were found to be normal. B) Quantification of the nptII transgene from the apical (AL) and basal leaves (BL) of the shoots a and b using real-time PCR. Values are means from six biological replicates.