Figure 5From: Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociationDissociation and Quantification of the chimerical tobacco line T17. A) Dissociation of the chimerical tobacco line T17 on rooting medium. Explants were transformed with the binary vector pBin19-35SGusintron carrying the nptII transgene for kanamycin selection and screened phenotypically. Shoots (a) in the left-hand jar were chosen for their poor root system, light green leaves and bleaching symptoms, whereas shoots (b) in the right-hand jar were found to be normal. B) Quantification of the nptII transgene from the apical (AL) and basal leaves (BL) of the shoots a and b using real-time PCR. Values are means from six biological replicates.Back to article page