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Figure 1 | BMC Biotechnology

Figure 1

From: Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation

Figure 1

Efficiency of amplification of npt II and β-actin genes in three independent DNA extractions from A) apricot callus and B) tobacco leaves, transformed uniformly, with the binary vector pBin19-35SGusintron. Both apricot callus and tobacco plants harboured one copy of the transgene. DNA samples from transgenic callus and plants were diluted serially to obtain a standard curve for both the endogenous β-actin and nptII genes and the Ct value was plotted against log [DNA]. Samples were run in three replicates of each of the three independent DNA extractions. The correlation coefficient and slope values are indicated.

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BMC Biotechnology

ISSN: 1472-6750

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