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Table 1 Summary of Agrobacterium expression constructs used during this study

From: Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice

Vector

Insert

clone name

Protein name

cell compartment targeted

pZPVX

SAG1 (full-length protein)

pZPVXSAG1a

PVXS

Cytoplasm

Pzp

SAG1 (full-length protein)

pApoSAG1a

AS

Apoplast

Pzp

nS (last 14 codons removed)

pAnS

AnS

Apoplast

Pzp

oS (last 14 codons removed and plant-optimized SAG1)

pAoS

AoS

Apoplast

Pzp

KnS (last 14 codons removed)

pKnS

KnS

Endoplasmic reticulum

Pzp

KoS (last 14 codons removed and plant-optimized SAG1)

pKoS

KoS

Endoplasmic reticulum

  1. a [13]