Humoral and cellular response in C3H/HeN vaccinated mice. (A) Determination of specific anti-rSAG1 humoral response in C3H/HeN mice. Serum IgG profile in immunized mice determined by ELISA. IgGt: a = p < 0.01: SAG1+Boost vs. Control+Boost; b = p < 0.001: SAG1+Boost vs. Control, SAG1 and naïve; IgG2a: a = p < 0.001 SAG1+Boost vs. Control, SAG1, Control+Boost and naïve; IgG1: b = p < 0.01 SAG1+Boost vs. Control, SAG1 and naïve. Values for each serum sample were determined in duplicate. (B) Delayed-type hypersensitivity (DTH) to Toxoplasma gondii 48 h post-intradermal injection in mice. a = p < 0.05: SAG1+Boost vs. Control+Boost; b = p < 0.01: SAG1+Boost vs. SAG1; c = p < 0.001: SAG1+Boost vs. Control and naïve. (C) Cytokine production by splenocytes from vaccinated mice. Cells were harvested two weeks after the last immunization and cultured in the presence of rSAG1 (10μg/ml). Supernatants were collected 72 h later and assessed for the production of IFN-γ by capture ELISA. a = p < 0.05: SAG1 and SAG1+Boost vs. Control and naïve. Control: mice vaccinated with pzp200-infiltrated leaf extracts, SAG1: pKnS-infiltrated leaf extracts, Control+Boost: pzp200-infiltrated leaf extracts + rSAG1 prime boost, SAG1+Boost: mice vaccine with pKnS-infiltrated leaf extracts plus rSAG1 prime boost. Results are expressed as the means value ± S.E.M and represent one of two similar experiments. Statistical analysis was performed by one-way analysis of variance (ANOVA) using the Bonferroni's Multiple Comparison Test.