Figure 2From: Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in miceSchematic representation of constructs used in the Agrobacterium -mediated transient expression systems. (A) Schematic representation of the expression cassette of intermediate plasmid pBPFΩ7 and the binary plant vector pZP200. (B) Schematic representation of pAn/oS and pKn/oS construct. An/oSAG1: the native and plant-optimized genes (residues 77 to 322, excluding the signal peptide in the N-terminus and the 14 hydrophobic amino acids in the C-terminus) fused to the AP24 osmotin signal peptide sequence in the N-terminus for driving the mature protein to the apoplast. Kn/oSAG1: the native and plant-optimized genes (residues 77 to 322) fused to the AP24 osmotin and an endoplasmic reticulum retention signal peptide sequences (TCTGAGAAAGATGAGCTAT) in the N-terminus and the C-terminus, respectively, for accumulation of the recombinant protein in the RE. 35sCaMVx2: duplicated CaMV 35S promoter; T-nos: nopaline synthase terminator sequence; Ω: translational enhancer sequence; bar: gluphosinate-resistant gene; RB and LB: right and left borders of the T region, respectively; MCS: multiple cloning site.Back to article page