Characteristics of the synthetic SAG1 gene. Nucleotides sequence of the native SAG1gene including the ClaI site at the start, and SmaI and EcoRI sites beyond the stop codons. The apoplastic and endoplasmic reticulum signal sequences at 5' and 3', respectively, are indicated in gray letters. The differences between the synthetic gene used in this study and the wild-type gene are denoted below the nucleotide sequence of the latter. The His-tag coding sequence is indicated as H. Gray boxes indicate consecutive A+T or G+C nucleotides of 5 bp or more.