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Table 3 Biomass and exobiopolymer production using a two-level fractional factorial design with 5 factors (2n-1).

From: Exobiopolymer production of Ophiocordyceps dipterigenaBCC 2073: optimization, production in bioreactor and characterization

No.

Glucose (g·L-1)

Malt extract (g·L-1)

Vitamin solution (mL·L-1)

Trace element solution (mL·L-1)

Temp. (°C)

Exobiopolymer (g·L-1)

Yield (g·g-1biomsdd)

Biomass (g·L-1)

1

20

6

0

0

30

7.2 ± 0.27

1.76 ± 0.03

4.1 ± 0.24

2

60

6

0

0

25

11.2 ± 0.21

2.67 ± 25

4.2 ± 0.63

3

20

14

0

0

25

11.2 ± 0.92

3.11 ± 0

3.6 ± 0.77

4

60

14

0

0

30

11.5 ± 0.25

2.40 ± 0.13

4.8 ± 0.42

5

20

6

2

0

25

9.1 ± 0.38

2.84 ± 0.08

3.2 ± 0.29

6

60

6

2

0

30

9.2 ± 0.23

1.44 ± 0.01

6.4 ± 0.21

7

20

14

2

0

30

6.4 ± 0.07

1.28 ± 0.04

5.0 ± 0.53

8

60

14

2

0

25

13.2 ± 0.74

2.70 ± 0.02

4.9 ± 0.15

9

20

6

0

2

25

10.2 ± 0.08

3.29 ± 0.14

3.1 ± 0.21

10

60

6

0

2

30

9.8 ± 0.60

1.72 ± 0.03

5.7 ± 0.07

11

20

14

0

2

30

6.7 ± 0.16

1.03 ± 0.04

6.5 ± 0.36

12

60

14

0

2

25

12.7 ± 0.09

2.44 ± 0.06

5.2 ± 0.50

13

20

6

2

2

30

4.8 ± 0.08

1.14 ± 0.23

4.2 ± 0.75

14

60

6

2

2

25

11.8 ± 0.62

2.27 ± 0.01

5.2 ± 0.90

15

20

14

2

2

25

11.7 ± 0.14

3.16 ± 0.04

3.7 ± 0.47

16

60

14

2

2

30

12.1 ± 0.35

1.83 ± 0.06

6.6 ± 0.49

17

40

10

1

1

27.5

12.1 ± 0.50

3.03 ± 20

4.0 ± 1.02

18

40

10

1

1

27.5

11.3 ± 0.48

2.90 ± 0.18

3.9 ± 0.68

19

40

10

1

1

27.5

11.4 ± 0.13

2.78 ± 0.04

4.1 ± 0.30

20

40

10

1

1

27.5

11.9 ± 0.20

3.13 ± 0.14

3.8 ± 1.21