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Table 2 Primers used in TNCL virus study

From: Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

Primer

Sequence (5'-3')

TNCL RNA segment

Position on viral genome*

Direction

Noda-R1-190F

gggaaccgagttacacgcgcattgc

1

190..214

>

Noda-R1-636R

ggtgaatggtgagtcagcatc

1

616..636

<

Noda-R1-1093F

caatctgtcaacgctaggcttatcgg

1

1093..1188

>

Noda-R1-1531R

ccgccctaagttgtagttgttgggacgg

1

1504..1531

<

Noda-R1-2368F

tgtaccgatgcgcttactccgttgatatcgg

1

2368..2398

>

Noda-R1-2933R

ccacgctgggtttctccagcagtgatgttacc

1

2902..2933

<

Noda-R2-269F

ggaatacctgatagatttgaaggcaaag

2

269..296

>

Noda-R2-810R

ggcaatgtttggataccctccaatatgtcg

2

781..810

<

Noda-D4

acatccagatccgatcaagt

2

491..510

>

Noda-U4

gccaggaatgttgcttgcaa

2

1161..1180

<

Primer

Sequence (5'-3')

gp64

Position*

direction

C-177Fs

attttggacgctgagggc

 

109139..109156

<

L-330Rs

cttgttgatgtgcgcatgcatcagctc

 

108731..108757

>

  1. *; position of each primer is based on sequences of deposited in GenBank under accession numbers EF690537 (TNCL virus RNA1), EF690538 (TNCL virus RNA2), and L22858 (AcMNPV)