SDS-PAGE analysis of SEAP production in SEAP-AcMNPV infected Ao38 cells. Samples of 20 μl from the supernatant of SEAP-AcMNPV infected Ao38 cells grown in Sf-900III medium were electrophoresed and major protein bands identified by Coomassie blue staining. Lane 1; protein marker sizes in kDa are indicated on the left. Lanes 2-3 represent two independent preparations of SEAP-AcMNPV infected culture medium (20 μl). Lane 4, SEAP-AcMNPV infected culture medium (20 μl) after centrifugation (80,520 × g for 60 min) to remove baculovirus virions. Lane 5, Control from WT AcMNPV infected culture medium (20 μl). An arrow indicates the position of the protein band that was excised and subjected to N-glycan analysis by LC-MS/MS.