Growth of Ao38 cells in culture. (A) The graph shows a one-step cell culture growth curve (solid line) of adherent cells. In addition, measurements of cell viability (dashed lines) are also included. Cell lines Sf9 (closed triangle), High Five (closed squares), and Ao38 (closed circles) grown in TNMFH medium were also compared with Ao38 cells grown in Sf-900III medium (open circles). All data points represent averages of triplicate samples and standard deviations are indicated by bars. (B) The graph shows a one step (dashed line) and a multi-step step (solid line) growth curve (open boxes) and viability (closed boxes) of suspension-culture Ao38 cells that were grown in Sf-900III medium in a shaker culture vessel at 100 rpm. Cells were diluted to approximately 0.25 × 106 cells/ml in fresh media on days 5 and 8. Data points represent the average of triplicate experiments and standard deviations are plotted.