Generation of Ascalapha odorata cell lines. Cell line development from a primary culture of Ascalapha odorata cells. Seventy-seven days after initiating primary cultures from A. odorata eggs, the Ao 2.0AA primary culture showed three cell foci containing proliferating cells. Left and right panels show Foci 1 and 2, respectively, which generated cells with distinct morphologies. The black bar represents 100 μm.