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Figure 5 | BMC Biotechnology

Figure 5

From: Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

Figure 5

Comparison of miR abundance in paired fresh-frozen and FFPE tissues, using different RNA extraction methods. Plots show the comparison of Ct values for three normal and three mantle cell lymphoma samples by the three different extraction methods: A) FFPE (RecoverAll) vs. mirVana; B) FFPE (RecoverAll) vs. TRIzol; C) TRIzol vs. mirVana. Red lines delineate the abundance strata for each extraction method, with numbers indicating the average number of miRs per sample in each zone. For example, in the FFPE vs. mirVana plot (Figure 5A), 44 miRs are high abundance by mirVana and medium abundance by FFPE (RecoverAll), but only 4 miRs are high abundance by FFPE (RecoverAll) and medium abundance by mirVana.

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BMC Biotechnology

ISSN: 1472-6750

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