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Figure 1 | BMC Biotechnology

Figure 1

From: Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

Figure 1

Ct values according to different input RNA concentrations. A) Plot of the difference in Ct values vs. mean Ct between duplicate plates for varying input RNA concentrations. Red lines indicate divisions between high (Ct<30), medium (30≤Ct≤35) and low (Ct>35) abundance strata. The total number of miRs is shown within each stratum for each RNA concentration (e.g., 72 miRs have Ct values <30 at the 10 ng RNA dilution compared to 112 miRs at the 200 ng dilution). B) Boxplots depicting the median of the absolute difference in Cts between duplicate plates for each miR abundance stratum according to input RNA concentration.

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BMC Biotechnology

ISSN: 1472-6750

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