Figure 2From: A modified fluorimetric host cell reactivation assay to determine the repair capacity of primary keratinocytes, melanocytes and fibroblastsRepair capacity of wildtype and XPA fibroblasts using different intensities for plasmid DNA irradiation. Cells were transfected with a mixture of irradiated DsRedexpress and pEGP-N1 in molar ratio 3:1. DsRed plasmids were irradiated with 1.25, 2.5, 5.0, 10.0 and 20 kJ/m² UVC prior to transfection. Repair capacity was calculated according to the results of FACS 36 h post transfection. The experiment was carried out in triplicates.Back to article page