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Figure 3 | BMC Biotechnology

Figure 3

From: Real-time detection of viable microorganisms by intracellular phototautomerism

Figure 3

Effect of phototautomeric probe structures on the RTV assay kinetics. The RTV assay was performed with live and heat-killed Staphylococcus aureus cells using (A) salicylic acid, (B) 2-hydroxy-cinnamic acid, and (C) 5-hydroxy-salicylic acid as probes. Optimal excitation and emission properties were selected for the probes as outlined in table 1. Probes were dissolved in 100 mM phosphate buffer pH 2 at a concentration of 2 mM.

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