Figure 5

α -AI1 expression in transgenic coffee beans from T0 plants. A. SDS-Polyacrylamide gel (15%) stained with Coomassie Brilliant Blue. M, Benchmark™ prestained protein ladder (Invitrogen); P, purified α-AI1 from Phaseolus vulgaris seeds; 1-3, total seed protein extract from transgenic coffee plants. The arrows indicate possible expressed α-AI1; 4, total seed protein extract of non-transgenic coffee plant. B. Western blot. M, Benchmark™ prestained protein ladder (Invitrogen); P, purified α-AI1 from Phaseolus vulgaris seeds; 1-3, total seed protein extract from transgenic coffee plants; 4, total seed protein extract of the non-transgenic coffee plant.