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Figure 3 | BMC Biotechnology

Figure 3

From: α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabicaplants inhibits α-amylases from the coffee berry borer pest

Figure 3

PCRs of positive C. arabica plants, showing npt II and α-AI1 amplifications PCRs from T0 (A and B) and T1 plants (C and D). A, 204-bp band from the α-AI1 gene of T0 plants. B, 411-bp band from the nptII gene of T0 plants. M, 1 kb plus leader; 1 - 6, T0 transformed coffee plants; 7, non-transgenic coffee plant; 8 positive control (pBIN19α-AI1 vector). C, 204-bp band from the α-AI1 gene of T1 plants. D, 411-bp band from the nptII gene of T1 plants. M, 1 kb plus leader; 1 - 11, positive T1 plants; 12, positive control (pBIN19α-AI1 vector); 13 non-transgenic coffee plant.

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BMC Biotechnology

ISSN: 1472-6750

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