Figure 4
From: Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins
Activity of CSK prepared using the BISOP method. Biotinylated Src protein was incubated with UBE2N, CSK purified using the BISOP method (eluted CSK and UBE2N), or commercially available GST-CSK fusion protein in the presence of cold (for immunoblot) or radioisotope-labeled (for autoradiogram) ATP. Src phosphorylation was assayed by immunoblot analysis using the anti-Phospho-Src (Y530) antibody. Autoradiogram shows phosphorylation of the Src protein by CSK.