Stable expression and phenotypic impact of attacin Etransgene in orchard grown apple trees over a 12 year period

Table 1 Segregation of the attacin E gene in 7 independent transformed apple lines determined by PCR analysis of progeny and number of T-DNA insertions determined by Southern analysis.

Lines	2000			2006			Southern Copy number
	Att negative	Att positive	ratio	Att negative	Att positive	ratio
TGx-157	17	10	1.7:1	46	30	1.5:1	2
TGx-158	13	15	1:1	51	49	1:1	1
TGx-161	21	19	1:1	-	-	-	1
TGx-164	12	11	1:1	-	-	-	1
TGx-165	7	6	1:1	-	-	-	1
TGx-168	25	12	2:1	-	-	-	2
TGx-178	21	24	1:1	42	33	1:1	1
'Galaxy'	35	0		75	0		0

ISSN: 1472-6750