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Figure 2 | BMC Biotechnology

Figure 2

From: A novel FRET pair for detection of parallel DNA triplexes by the LightCycler

Figure 2

Melting curves demonstrating pH and temperature dependence of ATTO, FAM and Cy5 fluorophores. (A, B) Fluorescence of ATTO495 and FAM at 530 nm from pH 4.5 to 7.5 (1 μM probe) in cacodylate buffer with MgCl2. (C, D) Fluorescence of ATTO495 and FAM at 530 nm from pH 4.5 to 5.75 in cacodylate buffer without MgCl2. (E) Fluorescence of Cy5 (decreasing by temperature) and ATTO647N (increasing by temperature) at 670 nm from pH 4.5 to 7.5 in sodium cacodylate buffer (1 μM probe).

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