Figure 2
From: The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation
GFP expression in genetically-modified trout muscle cells. Muscle cells cultivated at 18°C (A-C) or 10°C (D-F), 5 (A and D), 6 (B and E) and 7 days (C and F) post-seeding. At 18°C, GFP is visualized in fusing myogenic cells (day 5) and then in large myotubes (day 6 and 7). At 10°C, there is no GFP positive cell at day 5 and 6 of culture and only small myotubes express GFP at day 7. Nuclei were stained with Hoechst.