Figure 1
From: The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation
(A) Structure of the MLC2f-GFP transgene. This construct contains a myosin light chain (MLC2f) promoter at the 5', the GFP coding region, a fragment including the small t intron and polyadenylation signal from SV40 and a mylc 1/3 enhancer. The restriction sites for ligation are represented. The transgene is flanked by I-sceI sites so that it achieves I-SceI meganuclease-mediated transgenesis. (B-F) Expression of GFP in transgenic trout. (B) 50 somite stage embryo, lateral view: GFP labeling is present in most rostral somites and initially appears in medial domain of the somite (arrow). (C-E) Hatching stage embryo. (C) ventro-lateral view: GFP expression is present in myomeric axial musculature, (D, E) lateral view, bright field light (D) and fluorescence microscopy (E): GFP expression is detected in the head muscles (arrow). (F) Free swimming larvae. Transverse section: GFP is expressed in deep fast muscle fiber (FF) but not in superficial slow (SF) fibers. Scale bar = 500 μm in B, 2 mm in C, 350 μm in D and E and 40 μm in F.