Figure 6

Migration capacity of EGFP-expressing hUCB-MSCs. (A) hUCB-MSCs were transfected with the 2 μg of pEGFP-N1. After transfection, the migration response of hUCB-MSCs to conditioned medium from U-87MG cells or normal astrocytes was determined using a Transwell plate. Cell migration was compared and evaluated after staining by taking photographs and (B) counting cells that had migrated. Serum free medium was used as a negative control and SDF-1 was used as a positive control. The number of cells that had migrated to the lower side of the filter was counted in five or six different microscopic fields under a light microscope. (C) The expression of EGFP was observed in migrated hUCB-MSCs. Nuclei were counterstained with DAPI (blue). Experiments were performed in triplicate. Scale bar = 200 μm.