Figure 5

Multiple stem cell traits of EGFP-expressing hUCB-MSCs. (A) hUCB-MSCs were transfected with the 2 μg of pEGFP-N1 by microporation. After microporation, cells were cultured for additional 24 hr and analyzed with the trypan blue exclusion assay from 1-7 days. (B) Cells were labeled with antibodies against the indicated antigens and they were then analyzed by flow cytometry. hUCB-MSCs were transfected with the 2 μg of pEGFP-N1. After transfection, cells were allowed to differentiate under the specific induction medium. The accumulation of calcium-containing mineral deposits in the extracellular matrix and lipidic vacuoles were revealed by the (C) von Kossa staining method and (D) Oil Red O solution after 3 weeks. (E) EGFP expression was maintained in hUCB-MSCs during the osteoinductive conditions. The data are expressed as the mean ± SEM; n = 3. Scale bar (C) = 200 μm. Scale bar (D,E) = 100 μm.