Figure 6
The efficiency of microcell fusion correlated with the surface density of CD46 in recipient cells. (a)A given number of microcells (~8 × 105) were applied to different numbers of recipient cells and fused using MV or PEG. Abundant microcell hybrids, assessed as the number of drug-resistant colonies, were obtained using HT1080 and hiMSC cells. In contrast, few microcell hybrids were obtained using HFL-1 cells, irrespective of the fusogen used. (b) The expression level of CD46 on the cells was analyzed with flow cytometry by staining with FITC-conjugated anti-CD46 antibody (black peak) or an isotype control (white peak with solid line). No stained control was showed by white peak with dotted line. The numbers on the x and y axes represent fluorescence intensity and count of event, respectively. CD46 expression is indicated by the number within the graph which is the ratio of the mean fluorescence index of the black:white peaks.