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Figure 5 | BMC Biotechnology

Figure 5

From: Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer

Figure 5

The MV fusogen is more efficient than PEG for microcell hybrid production. Microcells collected from 24 flasks (~5 × 106) were fractionated into 5 dosing amounts (2 × 105, 4 × 105, 8 × 105, 1 × 106, 2 × 106) and added to (MV), or fused with (PEG), 2 × 106 of HT1080 cells in a 60-mm dish. On the following day, fused cells were plated onto two 100-mm dishes. Following selection culture for 14 days, drug-resistant colonies were stained with Giemsa and photographed.

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BMC Biotechnology

ISSN: 1472-6750

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