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Figure 1 | BMC Biotechnology

Figure 1

From: Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer

Figure 1

Rationale for microcell fusion using an MV fusogen. Donor CHO cells carry a human artificial chromosome (HAC) tagged with blastcidin-resistant (Bsr) and GFP gene. The CHO cells are transfected with plasmids encoding the MV-Fusion (MV-F) and Hemagglutinin (H) proteins and selection marker (Neo/DsRed). Microcells are prepared from the G418-resistant CHO donors and gave to recipient human cells. They are commonly coated with MV fusogen but contain different chromosomes. The donor-derived chromosome within the microcell is donated to the recipient cells by microcell fusion, which is mediated by interaction of the MV fusogen and the CD46 receptor presented on the surface of recipient cells. (a) The bsr-tagged HAC is rescued in mycrocell-hybrid by selection culture with Blasticidin. (b, c) On the other hand, introduction of the MV-H/F-tagged chromosome into recipient cells results in de novo synthesis of H/F proteins, leading to cell death caused by syncytium formation with the surrounding cells.

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