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Table 2 Microarray hybridization protocol used in an automated HS-400 hybridization station.

From: Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides

   Temp. C° Duration Repetitions
1 Prewash 85 Wash: 60 s; Soak: 30 s 1
2 Probe injection 34   1
3 Hybridization 34 High agitation 1
4 1. wash 23 Wash: 90 s; Soak: 30 s 3
5 2. wash 23 Wash: 90 s; Soak: 30 s 3
6 3. wash 23 Wash: 90 s; Soak: 30 s 3
7 Slide drying 23 90 s 1