Table 2 Microarray hybridization protocol used in an automated HS-400 hybridization station.
From: Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
Temp. C° | Duration | Repetitions | ||
|---|---|---|---|---|
1 | Prewash | 85 | Wash: 60 s; Soak: 30 s | 1 |
2 | Probe injection | 34 | 1 | |
3 | Hybridization | 34 | High agitation | 1 |
4 | 1. wash | 23 | Wash: 90 s; Soak: 30 s | 3 |
5 | 2. wash | 23 | Wash: 90 s; Soak: 30 s | 3 |
6 | 3. wash | 23 | Wash: 90 s; Soak: 30 s | 3 |
7 | Slide drying | 23 | 90 s | 1 |