Figure 4From: Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coliPurified total soluble mBCO (top panel) and its percentage as flavinylated covalent mBCO (bottom panel). Recombinant E. coli cells were grown at 37°C, added of 1 mM IPTG at OD600 nm = 1.5-2, and harvested after 18 hours at the temperature indicated below each bar. TB+20G: TB medium in the presence of 20 mL/L of glycerol: SB+8G; SB medium in the presence of 8 mL/L of glycerol. Data variability is <10%.Back to article page