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Figure 4 | BMC Biotechnology

Figure 4

From: Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli

Figure 4

Purified total soluble mBCO (top panel) and its percentage as flavinylated covalent mBCO (bottom panel). Recombinant E. coli cells were grown at 37°C, added of 1 mM IPTG at OD600 nm = 1.5-2, and harvested after 18 hours at the temperature indicated below each bar. TB+20G: TB medium in the presence of 20 mL/L of glycerol: SB+8G; SB medium in the presence of 8 mL/L of glycerol. Data variability is <10%.

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