Figure 2From: Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coliProduction of mBCO in 2 L-batch fermentation of recombinant E. coli cells under optimized conditions. Top panel: time course of pH (down triangle), pO2 (up triangle), temperature (square), and growth curve measured as OD600 nm (circle). Bottom panel: production of mBCO measured as specific activity (U/mg, filled bars) and volumetric productivity (U/L, empty bars). The arrow indicates the moment of IPTG addition.Back to article page